Archaeal DNA Polymerase-B as a DNA Template Guardian: Links between Polymerases and Base/Alternative Excision Repair Enzymes in Handling the Deaminated Bases Uracil and Hypoxanthine

Figure 3

Inhibition of DNA polymerase-D by DNA polymerase-B in the presence of template strand uracil. (a) Extension of a thymidine-containing (control) primer-template by Pol-D, Pol-B, and a mixture of Pol-D and Pol-B. (b) Extension of a uracil-containing primer-template by Pol-D, Pol-B, and a mixture of Pol-D and Pol-B. The numbers above the gel represent the extension time in minutes. In these experiments wild type (exo⁺) Pol-D and Pol-B were used. These experiments were carried out three times and in every case complete inhibition of Pol-D by Pol-B was noticed when uracil was present in the template.

(a) Thymidine

(b) Uracil