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Volume 2016, Article ID 4706532, 11 pages
Research Article

Discovery and Characterization of Iron Sulfide and Polyphosphate Bodies Coexisting in Archaeoglobus fulgidus Cells

1Department of Microbiology, Immunology, and Molecular Genetics, UCLA, Los Angeles, CA 90095, USA
2Electron Imaging Center for Nanomachines, California NanoSystems Institute, UCLA, Los Angeles, CA 90095, USA
3The UCLA Biomedical Engineering Interdepartmental Program, UCLA, Los Angeles, CA 09905, USA
4Institute of Industrial Biotechnology, GC University, Lahore 54000, Pakistan
5The UCLA-DOE Institute of Genomics and Proteomics, UCLA, Los Angeles, CA 90095, USA

Received 11 December 2015; Accepted 20 March 2016

Academic Editor: Harald Engelhardt

Copyright © 2016 Daniel B. Toso et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure S1: Negative stained A. fulgidus cell ghosts reveal “scars” along cell membrane. Arrows indicate the location of “scars” found in A. fulgidus cell ghosts.

Supplementary Figure S2: A. fulgidus cells in exponential growth phase showing newly developed granules. A,B) Slices from 3D tomograms reconstructed from cryoET tilt series of A. fulgidus whole cells in growth phase. The insets show the granules present within each cell at higher magnification.

Supplementary Figure S3: A. fulgidus development under varying nutritional conditions. A-E) TEM images of (A) a cell grown in normal conditions, (B) a cell grown with excess phosphate, (C) a cell grown with reduced phosphate, (D) a cell grown with reduced lactate, and (E) a cell grown with no phosphate. All samples were negatively stained with 1% UA.

Supplementary Figure S4: CryoEM of A. fulgidus cells after three generations of reduced or elevated phosphate conditions. A) Low magnification image of a TEM grid, stained with 1% UA, of a sample grown with no phosphate. Almost no cells are present. B-E) CryoEM images of cells grown in (B) 1 mM phosphate, (C) 2 mM phosphate, (D) 4 mM phosphate (normal conditions), and (E) 9 mM phosphate.

  1. Supplementary Figures