Characterization of the ATP-Dependent Lon-Like Protease in Methanobrevibacter smithii

Figure 2

Expression and purification of Lon-like-Ms from E. coli. Molecular mass standards are indicated at left. Lane 1, crude protein extract from noninduced cells; Lane 2, crude protein extract from IPTG-induced cells; Lane 3, soluble extract from IPTG-induced cells; Lane 4, unbound proteins eluted from the Ni-NTA column; Lane 5, proteins eluted with 5 mM imidazole; Lane 6, proteins eluted with 10 mM imidazole; Lane 7, proteins eluted with 300 mM imidazole.