Methanogen Diversity in Indigenous and Introduced Ruminant Species on the Tibetan Plateau

Table 1

Primer sets for pyrosequencing PCR and qPCR.

Name	Experiment	Target	Primer sequence (5′-3′)	Reference
A304f; A1000r; AbcL	Pyrosequencing	Total archaea	CGATTCATTAAAGCAGATCTCGATCCCCCCTAYGGGGYGCASCAG; GGCCATGCACYWCYTCTC; -bc; CGATTCATTAAAGCAGATCTCGATCCC	[11, 12]
RCC_762f; RCC_1099r	qPCR	Methanomassiliicoccaceae 1	GACGAAGCCCTGGGTC; GAGGGTCTCGTTCGTTAT	[13]
RCC_765f; RCC_892r	qPCR	Methanomassiliicoccaceae 2	GAAGCCCTRGGTCGCAAA; TACTCCCCAAGTRGCMGACTT	This study
Mbt_369f; Mbt_620r	qPCR	Methanobrevibacter	CCTCCGCAATGTGAGAAATCGC; TCWCCAGCAATTCCCACAGTT	This study

Bold: linker sequence; bc: barcode; italics: group-specific sequence; underline: linking sequence; double underline: 454 adaptor A; dotted underline: 454 adaptor B.