Discovering Antioxidant Molecules in the Archaea Domain: Peroxiredoxin Bcp1 from Sulfolobus solfataricus Protects H9c2 Cardiomyoblasts from Oxidative Stress

Figure 4

Transduction of TAT-Bcp1 in H9c2 cells. A western blot analysis of the cytoplasmic extracts was performed to detect TAT-Bcp1 in the H9c2 cells; the cytoplasmic proteins were analysed with a penta-His HRP-conjugated mAb. Actin was used as the cytoplasmic marker (lower panels). (a) H9c2 cells were cultured in growth medium (lane 1) and incubated with 0.5 μM (lane 2), 5 μM (lane 3), and 10 μM (lane 4) TAT-Bcp1 for 1 h. Purified TAT-Bcp1 was loaded as reference (lane 6). (b) H9c2 cells were cultured in growth medium (lane 6) and incubated with TAT-Bcp1 (10 μM) for 1 h (lane 7), 4 h (lane 8), 16 h (lane 9), and 24 h (lane 10). Increasing amounts of purified TAT-Bcp1 were loaded to construct a calibration curve to determine the intracellular TAT-Bcp1 levels: 25 ng (lane 1), 50 ng (lane 2), 75 ng (lane 3), and 100 ng (lane 4).

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