Discovering Antioxidant Molecules in the Archaea Domain: Peroxiredoxin Bcp1 from Sulfolobus solfataricus Protects H9c2 Cardiomyoblasts from Oxidative Stress

Figure 7

Effect of TAT-Bcp1 on cell apoptosis. Cell apoptosis was evaluated using EB and AO staining. H9c2 cells were treated with TAT-Bcp1 (10 μM) for different times (1 h, 4 h, 16 h, and 24 h) and then subjected to oxidative stress with 0.3 mM H₂O₂ for 1 h. (a) Representative images of the control cells (untreated with TAT-Bcp1) under growth and stress conditions showing both apoptotic (red) and live (green) cells. (b) The graph shows the mean percentages ± SD. of apoptotic cells. The grey and black bars represent the unstressed and stressed cells, respectively. versus the Ctrl growth condition; versus the Ctrl stress condition.

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