Discovering Antioxidant Molecules in the Archaea Domain: Peroxiredoxin Bcp1 from Sulfolobus solfataricus Protects H9c2 Cardiomyoblasts from Oxidative Stress

Figure 8

PARP-1 cleavage analysis. A western blot analysis of PARP-1 was performed using nuclear extracts. H9c2 cells were cultured under growth (lane 1) and stress conditions with 0.3 mM H₂O₂ for 3 h (lane 2) or pretreated with TAT-Bcp1 (10 μM) for different times, 1 h (lane 3), 4 h (lane 4), 16 h (lane 5), and 24 h (lane 6), before oxidative stress was induced (a). B23 was used as the nuclear marker (b). Both the 116 kDa full-length fragment and 89 kDa cleaved fragment are shown.

(a)

(b)