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| Crystals | Composition of protein solution | Composition of reservoir solution |
|
| Apo form (Apo) | 17.7 mg/mL apo form (0.32 mM of monomer) in 50 mM HEPES, pH 7.5, containing 50 mM NaCl and 5 mM β-ME | 0.1 М imidazole, pH 6.5, and 1.0 M sodium acetate trihydrate |
|
| AlDHPyr1147 as isolated (Holo-1) | 17 mg/mL AlDHPyr1147 as isolated in 50 mM HEPES, pH 7.5, containing 50 mM NaCl and 5 mM β-ME | 0.01 M nickel(II) chloride, 0.1 M Tris, pH 8.5, 1.4 M lithium sulfate, and 50 mM NaCl |
|
| Complex with NADP+ (Holo-2) | Cocrystallization of 17.7 mg/mL apo form with 27 mM NADP+ in 50 mM HEPES, pH 7.5, containing 50 mM NaCl and 5 mM β-ME | 0.1 М imidazole, pH 6.5, and 1.0 M sodium acetate trihydrate |
|
| Complex with NADP+ (Holo-3) | Soaking crystals of the apo form in excess of NADP+ and isobutyraldehyde for 40 min at 25°C |
|
| Ternary complex with NADP+ and substrate (ternary complex) | Cocrystallization of 17.7 mg/mL apo form with 27 mM NADP+ and 400 mM isobutyraldehyde in 50 mM HEPES, pH 7.5, containing 50 mM NaCl and 5 mM β-ME | 0.1 М imidazole, pH 6.5, and 1.0 M sodium acetate trihydrate |
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