Construction of Expression Shuttle Vectors for the Haloarchaeon Natrinema sp. J7 Based on Its Chromosomal Origins of Replication

Figure 2

Structural stability of the shuttle vectors in J7-F cells. Pretransformed plasmids (extracted from E. coli before being transformed into J7-F, lanes 1 and 2) and back-transformed plasmid (extracted from E. coli following transformation into J7-F, lanes 3 and 4) were digested with restriction enzymes to compare their structural stabilities. M, 2 kb plus II marker; lanes 1 and 3, undigested plasmids; lanes 2 and 4, plasmids digested with SnaBI and MfeI.