Construction of Expression Shuttle Vectors for the Haloarchaeon Natrinema sp. J7 Based on Its Chromosomal Origins of Replication

Figure 3

Maintenance of the pFJ1, pFJ4, and pFJ6 shuttle vectors in J7-F cells. J7-F cells containing different shuttle vectors (50 μL) were diluted in 5 mL Halo-2 medium and grown for 24 h. For every two dilutions (12 dilutions in total), aliquots were spread onto Halo-2 plates, and 50 random colonies were selected and spotted onto nonselective 18% MGM plates. Maintenance was measured by calculating the percentage of colonies growing on selective 18% MM plates.