Catalytic Intermediate Crystal Structures of Cysteine Desulfurase from the Archaeon Thermococcus onnurineus NA1

Figure 2

Structure of native ToCDS (NAT) in crystal I. (a) Overall internal aldimine structure of ToCDS dimer. (b) Protomer structure of ToCDS. (c) Active site structure of ToCDS. Substrate cysteine-binding site is shown as an orange shade. Omit maps of internal aldimine of PLP and Lys216 are shown. The omit maps show the 2Fo-Fc electron density map (contoured at 1.0 σ; blue) and Fo-Fc electron density map (contoured at 3.0 σ; green).

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