Research Article
A Flexible System for Cultivation of Methanococcus and Other Formate-Utilizing Methanogens
| Component | For tubes | For 1-liter bottle | For 100 mL | For 1000 mL |
| Glass-distilled water | 30 mL | 300 mL | Glycylglycine buffer, 1 M, pH = 8.0 | 20 mL | 200 mL | General salt solution | 50 mL | 500 mL | K2HPO4, 14 g/L | 1.0 mL | 10 mL | Na acetate·3H2O, 136 g/L | 1.0 mL | 10 mL | Trace mineral solution [27] | 1.0 mL | 10 mL | Iron stock solution [35] | 0.5 mL | 5 mL | Resazurin, 0.1 g/100 mL | 0.1 mL | 1 mL | Sodium formate (NaCOOH) | 2.7 g | 27 g | Sodium bicarbonate (NaHCO3) | 0.5 g | 5.0 g | Casamino acids (for complex medium) | 0.5 g | 5.0 g | Alanine (optional, 100 mM) | 1.0 mL | 10 mL |
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aMedium components are based upon Balch and Wolfe [35], Romesser et al. [36], and Whitman et al. [27].
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