A Flexible System for Cultivation of Methanococcus and Other Formate-Utilizing Methanogens

Table 3

Component	For tubes	For 1-liter bottle
	For 100 mL	For 1000 mL
Glass-distilled water	10 mL	100 mL
Glycylglycine buffer, 1 M, pH = 6.5	40 mL	400 mL
General salt solution	50 mL	500 mL
K2HPO4, 14 g/L	1.0 mL	10 mL
Na acetate·3H2O, 136 g/L	1.0 mL	10 mL
Trace mineral solution	1.0 mL	10 mL
Iron stock solution	0.5 mL	5 mL
Resazurin, 0.1 g/100 mL	0.1 mL	1 mL
Sodium formate (NaCOOH)	2.7 g	27 g
Sodium bicarbonate (NaHCO3)	0.5 g	5.0 g
Casamino acids (for complex medium)	0.5 g	5.0 g