Development of the Multiple Gene Knockout System with One-Step PCR in Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius

Figure 7

Growth curve of the argD deletion mutant SK-5. Wt (SK-1) and ΔargD (SK-5) were cultivated in XTU liquid medium with or without agmatine at 75°C with shaking. Closed circles: SK-5 with agmatine (100–1000 μg/mL); open circles: SK-5 without agmatine; open squares: SK-1 without agmatine. Error bars represent the standard deviation from three independent experiments.