Production of Poly(3-Hydroxybutyrate) by Haloarcula, Halorubrum, and Natrinema Haloarchaeal Genera Using Starch as a Carbon Source
Table 2
Screening of PHA-producing archaeal strains using phenotypic and genotypic methods.
Sample type
Name of isolates
Morphology
Colony staining method
CODEHOP PCR
ARDRA
SBB
NR
PhaE
PhaC
Profiles
Water and sediments (2014)
CEJ1-14
Cocci
-
-
-
-
I
CEJ2-14
Cocci
-
-
-
-
II
CEJ3-14
Cocci
+
+
+
+
III
CEJ4-14
Cocci
-
-
-
-
I
CEJ5-14
Cocci
+
+
+
+
III
CEJ6-14
Cocci
+
+
+
+
III
CEJ7-14
Cocci
+
+
+
+
III
CEJ8-14
Cocci
+
+
+
+
III
CEJ9-14
Cocci
+
+
+
+
III
CEJ10-14
Cocci
+
+
+
+
III
CEJ11-14
Cocci
+
+
+
+
III
CEJ17-14
Pleomorph
-
-
-
-
I
CEJ18-14
Pleomorph
-
-
-
-
I
CEJ21-14
Cocci
+
+
+
+
III
CEJ24-14
Cocci
+
+
+
+
III
CEJ25-14
Cocci
+
+
+
+
III
CEJ26-14
Cocci
-
-
-
-
I
CEJ28-14
Cocci
+
+
+
+
III
CEJ29-14
Short rod
-
-
-
-
I
CEJ32-14
Cocci
-
-
-
-
II
Water (2014)
CEJ33-14
Cocci
-
-
-
-
I
CEJ34-14
Cocci
+
+
+
+
I
Water and sediments (2010)
CEJ35-10
Cocci
-
-
-
-
IV
CEJ36-10
Cocci
-
-
-
-
IV
CEJ37-10
Cocci
-
-
-
-
IV
CEJ38-10
Cocci
-
-
-
-
IV
CEJ41-10
Cocci
-
-
-
-
VI
CEJ42-10
Cocci
-
-
-
-
VI
CEJ43-10
Cocci
-
-
-
-
IV
CEJ45-10
Cocci
-
-
-
-
IV
CEJ46-10
Cocci
-
-
-
-
IV
CEJ47-10
Cocci
-
-
-
-
I
CEJ48-10
Cocci
+
+
+
+
V
Designation of isolates by letters indicating first the origin of strains from Chott El Jerid (CEJ) followed by the number of the isolates, then a number indicating the year of sampling. The ARDRA pattern is indicated by Roman numeral. +, detectable; -, not detectable; SBB: Sudan Black B; NR: Nile Red.
