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Research Article

Production of Poly(3-Hydroxybutyrate) by Haloarcula, Halorubrum, and Natrinema Haloarchaeal Genera Using Starch as a Carbon Source

Table 2

Screening of PHA-producing archaeal strains using phenotypic and genotypic methods.

Sample typeName of isolatesMorphologyColony staining methodCODEHOP PCRARDRA
SBBNRPhaEPhaCProfiles

Water and sediments (2014)CEJ1-14Cocci----I
CEJ2-14Cocci----II
CEJ3-14Cocci++++III
CEJ4-14Cocci----I
CEJ5-14Cocci++++III
CEJ6-14Cocci++++III
CEJ7-14Cocci++++III
CEJ8-14Cocci++++III
CEJ9-14Cocci++++III
CEJ10-14Cocci++++III
CEJ11-14Cocci++++III
CEJ17-14Pleomorph----I
CEJ18-14Pleomorph----I
CEJ21-14Cocci++++III
CEJ24-14Cocci++++III
CEJ25-14Cocci++++III
CEJ26-14Cocci----I
CEJ28-14Cocci++++III
CEJ29-14Short rod----I
CEJ32-14Cocci----II

Water (2014)CEJ33-14Cocci----I
CEJ34-14Cocci++++I

Water and sediments (2010)CEJ35-10Cocci----IV
CEJ36-10Cocci----IV
CEJ37-10Cocci----IV
CEJ38-10Cocci----IV
CEJ41-10Cocci----VI
CEJ42-10Cocci----VI
CEJ43-10Cocci----IV
CEJ45-10Cocci----IV
CEJ46-10Cocci----IV
CEJ47-10Cocci----I
CEJ48-10Cocci++++V

Designation of isolates by letters indicating first the origin of strains from Chott El Jerid (CEJ) followed by the number of the isolates, then a number indicating the year of sampling. The ARDRA pattern is indicated by Roman numeral. +, detectable; -, not detectable; SBB: Sudan Black B; NR: Nile Red.