Also in Crayfish: How Phytase Inclusion Avoids Phytic Acid Effects on Hepatopancreas Enzymes of Redclaw Cherax quadricarinatus

Table 2

Remaining enzymatic activities after four hours of in vitro digestive incubation of Cherax quadricarinatus (n = 3). Values are expressed as means ± SD.

Remaining Activities (U/ml)	Phytase (PU/kg DM)	PA level (%)			SEM	-value
		0.56	1.68	2.80		PA level	Phy level	PA × Phy
Alkaline protease	0	28.92 ± 0.98^Ac	26.32 ± 0.74^Bc	23.23 ± 0.93^Cc	0.23	<0.0001	<0.0001	0.2049
	250	30.83 ± 0.27^Ab	29.33 ± 0.49^Bb	26.40 ± 0.60^Cb
	500	31.89 ± 0.91^Aa	30.77 ± 0.57^Ba	28.13 ± 0.34^Ca
α-Amylase	0	5.42 ± 0.10^ab	4.52 ± 0.08^def	4.22 ± 0.12^f	0.10	<0.0001	0.0001	0.0402
	250	5.49 ± 0.08^a	4.85 ± 0.39^cde	4.44 ± 0.24^df
	500	5.53 ± 0.10^a	5.11 ± 0.03^abc	4.97 ± 0.13^bcd
Cellulase	0	8.13 ± 0.40	8.09 ± 0.37	7.36 ± 0.12	0.24	0.0683	0.7603	0.6837
	250	8.06 ± 0.34	8.03 ± 0.57	7.68 ± 0.58
	500	7.93 ± 0.44	7.72 ± 0.27	7.68 ± 0.47

^a–cDifferent lowercased letters indicate a significant effect of phytase dose. ^A–CDifferent uppercased letters indicate a significant effect of phytic acid level. U, units; PU, phytase units; DM, dry matter; PA, phytic acid; Phy, phytase; SEM, standard error of the mean.