Characterization of the Virulency of Vibrio spp. in Giant Tiger Shrimp (Penaeus monodon) from Peninsular Malaysia

Figure 5

Transversal view of hepatopancreas sections of non-infected (a) and infected (b–f) shrimp (H&E). (a) Normal appearance of hepatopancreas tubules observed in unchallenged shrimp. The tubules are lined by viable R and B cells, while the lumen is large and clear (L). (H&E, bar = 50 µm). (b) Tubular epithelium lacked intracytoplasmic vacuolation, exhibiting intense eosinophilic cytoplasm. Note that the epithelial cells are elongated, producing prominence into the lumen (black arrow) (H&E, bar = 50 µm). (c) Necrosis (dash arrow) and sloughing of tubular epithelial cells (black arrow), leading to presence of cellular debris in the partially occluded tubular lumen. The number of vacuoles in the affected R and B cells are generally low (H&E, bar = 50 µm). (d) Observation of sloughed tubular epithelial cells in lumen of hepatopancreatic tubules and enlargement of nucleus (karyomegaly) (black arrow) (H&E, bar = 50 µm). (e) Infiltration of hemocytes infiltration (black arrow) in the interstitial space (H&E, bar = 50 µm). (f) Massive vacuolation (black arrow) (H&E, bar = 50 µm). HP, hepatopancreas.

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