Characterization of the Virulency of Vibrio spp. in Giant Tiger Shrimp (Penaeus monodon) from Peninsular Malaysia

Table 1

Virulence factors, sequence of primers, references, and expected amplicon size of target gene used in this study.

Gene	Virulence factor	Primer sequence (5^′–3^′)	Amplicon size (bp)	References
pirA	AHPND strains of V. parahaemolyticus	F: TGACTATTCTCACGATTGGACTG	284	Han et al. [16]
		R: CACGACTAGCGCCATTGTTA
pirB	AHPND strains of V. parahaemolyticus	F: TGATGAAGTGATGGGTGCTC	392	Han et al. [16]
		R: TGTAAGCGCCGTTTAACTCA
chiA	Chitinase	F: GGAAGATGGCGTGATTGACT	232	Ruwandeepika et al. [17]
		R: GGCATCAATTTCCCAAGAGA
luxR	Quorum sensing factors	F: ATGGACTCAATTGCAAAGAG	618	Ruwandeepika et al. [17]
		R: TTAGTGATGTTCACGGTTGT
flaC	Flagella	F: AAATCATTCCAAATCGGTGC	580	Bai et al. [18]
		R: TCTTTGATTCGGCTCTTA
hlyA	Hemolysin	F: GGCAAACAGCGAAACAAATACC	738	Saravanan et al. [19]
		R: CTCAGCGGGCTAATACGGTTTA
toxR	Transmembrane transcription regulator	F: ATACGAGTGGTTGCTGTCATG	368	Letchumanan et al. [20]
		R: GTCTTCTGACGCAATCGTTG
ctxA	Cholera toxin	F: CGGGCAGATTCTAGACCTCCTG	563	Sechi et al. [21]
		R: CGATGATCTTGGAGCATTCCCAC
tlh	Thermolabile hemolysin	F: AAAGCGGATTATGCAGAAGCACTG	450	Bej et al. [22]
		R: GCTACTTTCTAGCATTTTCTCTGC
tdh	Thermostable direct hemolysin (TDH)	F: GTAAAGGTCTCTGACTTTTGGAC	269	Bej et al. [22]
		R: TGGAATAGAACCTTCATCTTCACC
trh	TDH-related hemolysin (TRH)	F: TTGGCTTCGATATTTTCAGTATCT	500	Bej et al. [22]
		R: CATAACAAACATATGCCCATTTCCG