The Unusual Circulation of the Newt Heart after Ventricular Injury and Its Implications for Regeneration
Bleeding from the heart after needle puncture. (a) An intact newt ventricle and (b) a ventricle that is surrounded by blood. (c) Location of the needle-stick in the heart (arrow “b”). Arrow “a” indicates a coagulated clot. Sections (a–c) were processed with Masson’s trichrome stain. (d) A ventricle that is surrounded by phagocytes (HE stain). (e) Collagen fibrogenesis of cardiomyocytes after needle puncture (trichrome stain). The collagenous fibers are stained blue. (f) Expression of LC3, a protein associated with autophagic cell death. LC3 protein that is conjugated with HRP-black is observed (arrows). Magnification: ((a), (b), and (d)) ×40; ((c), (e), and (f)) ×100.