Table of Contents
Anatomy Research International
Volume 2012, Article ID 948704, 5 pages
Review Article

Ultrahigh Voltage Electron Microscopy Links Neuroanatomy and Neuroscience/Neuroendocrinology

1Laboratory of Neuroendocrinology, Ushimado Marine Institute, Graduate School of Natural Science and Technology, Okayama University, Kashino, Ushimado, Setouchi, Okayama 701-4303, Japan
2Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan

Received 26 April 2011; Accepted 15 September 2011

Academic Editor: Ilkan Tatar

Copyright © 2012 Hirotaka Sakamoto and Mitsuhiro Kawata. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The three-dimensional (3D) analysis of anatomical ultrastructures is extremely important in most fields of biological research. Although it is very difficult to perform 3D image analysis on exact serial sets of ultrathin sections, 3D reconstruction from serial ultrathin sections can generally be used to obtain 3D information. However, this technique can only be applied to small areas of a specimen because of technical and physical difficulties. We used ultrahigh voltage electron microscopy (UHVEM) to overcome these difficulties and to study the chemical neuroanatomy of 3D ultrastructures. This methodology, which links UHVEM and light microscopy, is a useful and powerful tool for studying molecular and/or chemical neuroanatomy at the ultrastructural level.