|
| OA Related Genes |
| Gene name | Gene abbreviation | Sequence (5′ 3′) | Annealing temp | Amplicon length | NCBI Number |
|
| Cartilage matrix | | | | | |
| Collagen, Type I, Alpha 1 | Col1a1 | F: CAACCGCTTCACCTACAGC | 60 | 101 | AK236626 |
| R: TTTTGTATTCGATCACTGTCTTGCC |
| Collagen, Type II, Alpha 1 | Col2a1 | F: GAGAGGTCTTCCTGGCAAAG | 60 | 118 | AF201724.1 |
| R: AAGTCCCTGGAAGCCAGAT |
| Aggrecan | Acan | F: TGCAGGTGACCATGGCC | 60 | 79 | AF201722b |
| R: CGGTAATGGAACACAACCCCT |
| SRY (sex determining gene region Y) box-9 | Sox9 | F: CAGGGCTCTGTGCTCTACTCC | 60 | 230 | NM_213843.1 |
| R: GGGTTACGGTCTTTCTTCGGT |
| Osteopontin | Opn | F: CCGCAGCCAGGAGCAGTC | 55 | 214 | NM_214023.1 |
| R: GTTGATCTCAGAAGACGCACTCTC |
| Cartilage oligometric matrix protein | Comp | F: GGCTGGAAGGACAAGACATC | 55 | 82 | XM_003123529.1 |
| R: CCTCATAGAACCGCACTCTG |
|
| Degradative enzymes & inhibitors | | | | | |
| Matrix metalloprotease-1 | Mmp1 | F: TGATGGACCTGGAGGAAACC | 59 | 131 | NM_001166229 |
| R: GAGCAGCCACACGATACAAG |
| Matrix metalloprotease-3 | Mmp3 | F: GATGTTGGTTACTTCAGCAC | 50 | 197 | NM_001166308.1 |
| R: ATCATTATGTCAGCCTCTCC |
| Matrix metalloprotease-13 | Mmp13 | F: CCAAAGGCTACAACTTGTTTCTTG | 60 | 77 | AF069643 |
| R: TGGGTCCTTGGAGTGGTCAA |
| TIMP metallopeptidase inhibitor-1 | Timp1 | F: CCTCGTACCAGCGTTATG | | | |
| R: CGTTCCACAGTTGTCCAG | 59 | 177 | NM_213857.1 |
| TIMP metallopeptidase inhibitor-2 | Timp2 | F: ATATACGAGAACACCAGACC | | | |
| | R: GGAATGATTACAACGGATGC | 59 | 152 | AK237154.1 |
| ADAM metallopeptidase with thrombospondin Type 1 motif 5 | Adamts5 | F: CGCTGCCACCACACTCAA
R: CGTAGTGCTCCTCATGGTCATCT | 60 | 80 | NM_007038.3 |
|
| Inflammatory response | | | | | |
| Indian hedgehog | Ihh | F: CAGCGGGCGCTATGAAGGCA | 60 | 140 | XM_001925486.1 |
| R: GGTCCTTGCAGCGCTGGGTC |
| Transforming growth factor β | Tgfb | F: GGAGTGGCTGTCCTTTGATGT | 60 | 117 | NM_214015.1 |
| R: AGTGTGTTATCTTTGCTGTCA |
| Nitric oxide synthase 2, inducible | Inos | F: TGAATTTGTCAACCTGTATTAC | 53 | 82 | NM_001143690.1 |
| R: CTTTGTTACCGCTTCCAC |
| Chitinase-3-like protein 1 | Chi3l1 | F: TGACGCTCTATGACACAC | 62 | 194 | NM_001001540 |
| R: GGCTAGGTCCAGTCCATC |
|
| Cell proliferation & apoptosis | | | | | |
| Caspase-8 | Casp8 | F: TGGGCAAACAGATGCCACAACCT | 60 | 153 | NM_001031779.2 |
| R: CCCCTTCAATCTAGCCCACCCCC |
| Fas (TNF receptor superfamily, member 6) | Fas | F: TAGAGTTTGTGATGGAGAA | 53 | 107 | NM_213839.1 |
| R: ATTGAGAAGTGTGACAGA |
|
