RNA Interference Mediated Interleukin-1β Silencing in Inflamed Chondrocytes Decreases Target and Downstream Catabolic Responses

Figure 5

Quantitative polymerase chain reaction (qPCR) data showing % change in gene expression of inflammatory mediators in control, untransduced (C), LPS-stimulated (LPS), rAAV2-tdT-shIL-1β transduced (shIL-1β), and rAAV2-tdT-shIL-1β transduced and LPS-stimulated chondrocytes (shIL-1β + LPS). % change (±SEM) is shown relative to LPS chondrocytes. Expression of (a) RELA (p65) and (b) IL-6 was significantly increased with LPS stimulation. The expression of both (a) RELA (p65) and (b) IL-6 was highest in shIL-1β + LPS chondrocytes. Data were analyzed using ANOVA followed by Tukey’s post hoc pairwise analysis. Graph bars with different Tukey classification letters are significantly different at .

(a)

(b)