RNA Interference Mediated Interleukin-1β Silencing in Inflamed Chondrocytes Decreases Target and Downstream Catabolic Responses

Figure 7

Quantitative polymerase chain reaction (qPCR) data showing % change in gene expression of ECM proteins in control, untransduced (C), LPS-stimulated (LPS), rAAV2-tdT-shIL-1β transduced (shIL-1β), and rAAV2-tdT-shIL-1β transduced and LPS-stimulated chondrocytes (shIL-1β + LPS). % change (±SEM) is shown relative to LPS chondrocytes. (a) COL2A1 and (b) ACAN are shown. Both COL2A1 expression and ACAN expression are decreased with LPS stimulation and this is not rescued with rAAV2-tdT-shIL-1β transduction. Data were analyzed using ANOVA followed by Tukey’s post hoc pairwise analysis. Graph bars with different Tukey classification letters are significantly different at .

(a)

(b)