RNA Interference Mediated Interleukin-1β Silencing in Inflamed Chondrocytes Decreases Target and Downstream Catabolic Responses

Table 2

Equine primer sequences used to analyze gene expression.

18S	Forward, 5′-CGGCTTTGGTGACTCTAGATAACC-3′
	Reverse, 5′-CCATGGTAGGCACAGCGACTA-3′
IL-1β	Forward, 5′-CGTCTCCCAGAGCCAATCC-3′
	Reverse, 5′-CACCAGGCTGACTTTGAGTGAGT-3′
TNF-α	Forward, 5′-CAGCCGCTTAGCTGTCTCCTA-3′
	Reverse, 5′-GTGTGGCAAGGGCTCTTGAT-3′
ADAMTS4	Forward, 5′-CCCTGGTCTCCGAAACCTCTA-3′
	Reverse, 5′-TATTCACCATTGAGGGCATAGGA-3′
ADAMTS5	Forward, 5′-CAGACGTTGGGACCATATGCT-3′
	Reverse, 5′-TGCGTGGAGGCCATCAT-3′
MMP3	Forward, 5′-CTTATCAAAAATGGCTGCGTCTATT-3′
	Reverse, 5′-GCAGAGACAGTGTTTTCATTTTTAAG-3′
MMP13	Forward, 5′-TGAAGACCCGAACCCTAAACAT-3′
	Reverse, 5′-GAAGACTGGTGATGGCATCAAG-3′
IL-6	Forward, 5′-AGTAACCACCCCTGACCCAACT-3′
	Reverse, 5′-TGTTGTGTTCTTCAGCCACTCA-3′
RELA (p65)	Forward, 5′-GCTTATGGATTCTGAGGGTGTGT-3′
	Reverse, 5′-CCAAAAGGATATAGATACTGCCAATAAA-3′
Collagen type II	Forward, 5′-CGCTGTCCTTCGGTGTCA-3′
	Reverse, 5′-CTTGATGTCTCCAGGTTCTCCTT-3′
Aggrecan	Forward, 5′-GATGCCACTGCCACAAAACA-3′
	Reverse, 5′-GATGCCACTGCCACAAAACA-3′

18S, 18 small ribonucleic acid; IL, interleukin; TNF-α, tumor necrosis factor-α; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; MMP, matrix metalloproteinase.