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Volume 2016 (2016), Article ID 4019873, 10 pages
Research Article

Infrapatellar Fat Pad: An Alternative Source of Adipose-Derived Mesenchymal Stem Cells

1Department of Orthopaedics, Faculty of Medicine, Ramathibodi Hospital, Bangkok 10400, Thailand
2Office of Research and Innovation, Ramathibodi Hospital, Bangkok 10400, Thailand
3Department of Surgery, Faculty of Medicine, Ramathibodi Hospital, Bangkok 10400, Thailand
4Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Bangkok 10400, Thailand
5Department of Orthopaedics, Royal Infirmary Edinburgh Hospital, Edinburgh University, Edinburgh, UK

Received 25 November 2015; Revised 7 February 2016; Accepted 14 February 2016

Academic Editor: Changhai Ding

Copyright © 2016 P. Tangchitphisut et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Introduction. The Infrapatellar fat pad (IPFP) represents an emerging alternative source of adipose-derived mesenchymal stem cells (ASCs). We compared the characteristics and differentiation capacity of ASCs isolated from IPFP and SC. Materials and Methods. ASCs were harvested from either IPFP or SC. IPFPs were collected from patients undergoing total knee arthroplasty (TKA), whereas subcutaneous tissues were collected from patients undergoing lipoaspiration. Immunophenotypes of surface antigens were evaluated. Their ability to form colony-forming units (CFUs) and their differentiation potential were determined. The ASCs karyotype was evaluated. Results. There was no difference in the number of CFUs and size of CFUs between IPFP and SC sources. ASCs isolated from both sources had a normal karyotype. The mesenchymal stem cells (MSCs) markers on flow cytometry was equivalent. IPFP-ASCs demonstrated significantly higher expression of SOX-9 and RUNX-2 over ASCs isolated from SC (6.19 ± 5.56-, 0.47 ± 0.62-fold; value = 0.047, and 17.33 ± 10.80-, 1.56 ± 1.31-fold; value = 0.030, resp.). Discussion and Conclusion. CFU assay of IPFP-ASCs and SC-ASCs harvested by lipoaspiration technique was equivalent. The expression of key chondrogenic and osteogenic genes was increased in cells isolated from IPFP. IPFP should be considered a high quality alternative source of ASCs.