Table of Contents
Advances in Toxicology
Volume 2014, Article ID 371497, 11 pages
http://dx.doi.org/10.1155/2014/371497
Research Article

Differential Effect of Isooctane Doses on HaCaT and HeLa: A Multimodal Analysis

1School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India
2Department of Biochemistry, Ballygunge Science College, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India

Received 4 July 2014; Accepted 10 September 2014; Published 9 October 2014

Academic Editor: Sung Y. Eum

Copyright © 2014 Lopamudra Das et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A multimodal approach is effective in analyzing biological problems critically and thus also useful in assessing cytotoxicity under chemicals assaults. In this study effects of isooctane, an organic solvent and component of gasoline produced in petroleum industries, have been explored on normal (HaCaT) and cancerous (HeLa) epithelial cells. Besides morphological alterations, impacts on viability, prime molecular expressions, and bioelectrical properties on exposure to different doses of isooctane were noted. Scanning electron microscopy and viability assay demonstrated remarkable structural alterations and cell death, respectively, in HaCaT but not in HeLa. Transcriptomic and immunocytochemical studies on E-cadherin expression also elucidated pronounced toxic effects on HaCaT. Remarkable changes on the bioelectrical properties (e.g., impedance and phase angle) of the HaCaT, in contrast to HeLa, at different temporal points on isooctane exposure also indicated cytotoxic effects in the former. Hence this study illustrated cytotoxicity of isooctane on HaCaT multidimensionally which was evaded by HeLa.