Intracellular and Extracellular Redox Status and Free Radical Generation in Primary Immune Cells from Children with Autism

<table>Intracellular Free Radicals are Elevated in Lymphocytes from Children with Autism. Intracellular free radicals were measured in freshly isolated PBMC from children with autism and unaffected control children using 1 uM DCF. Presented is median fluorescent intensity (MFI) of the gated lymphocyte population from subject samples normalized to MFI of a standard PBMC preparation also treated with 1 uM DCF and analyzed with each subject sample. Lymphocytes from children with autism exhibited a significantly higher mean level of intracellular free radicals than controls (<svg height="11.375" id="M97" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>). Control median (95% CI) = 0.576 (0.551–0.640); case median (95% CI) = 0.689 (0.561–1.086).</table>

Autism Research and Treatment

fig2

Figure 2

Figure 2: Intracellular and Extracellular Redox Status and Free Radical Generation in Primary Immune Cells from Children with Autism 