A Need for Consistency in Behavioral Phenotyping for ASD: Analysis of the Valproic Acid Model

Table 4

Repetitive behavior in open environments.

Reference	Apparatus	Pretest habituation	Duration	Coding methods	Behaviors analyzed	Outcome
Gandal et al. [10]	Cage without bedding	20 min to new cage	10 min	Manual real-time (blind)	Time spent grooming	VPA increased total grooming time
Kim et al. [12]	Typical cage	10 min to new cage	10 min	Real-time 2 meters from cage	Grooming and digging	VPA increased digging. No difference for grooming
Kang and Kim [14]	Fresh bedded home cage in novel environment	72-hour isolation	10 min	Manual from recording	Grooming time, jumping bouts, digging	VPA increased grooming time and jumping bouts
Kang and Kim [14]	48-hour movements in familiar environment	48-hour isolation	48 hours	Vibration sensitive plate LABORAS cage	Distance moved, grooming time, total rearing time	No difference between VPA and saline
Campolongo et al. [16]	Clear plexiglass cylinder	1-hour habituation for 2 days before testing	10 min	Manual from recording, condition blind	Grooming of all body regions	Effect of VPA treatment on grooming