Table of Contents
Advances in Zoology
Volume 2014, Article ID 258134, 14 pages
http://dx.doi.org/10.1155/2014/258134
Research Article

Morphological and Molecular Identification of Three Ceriodaphnia Species (Cladocera: Daphniidae) from Australia

School of Earth and Environmental Sciences, University of Adelaide, Adelaide, SA 5000, Australia

Received 6 August 2014; Revised 14 October 2014; Accepted 16 October 2014; Published 14 December 2014

Academic Editor: Lorena Rebecchi

Copyright © 2014 Pranay Sharma. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Australian Ceriodaphnia (Cladocera: Daphniidae) are examined using morphological attributes and two mitochondrial DNA (COI and 16s) and one nuclear DNA (28s) gene fragments to differentiate the species. The sequence data supports the existence of three species, that is, C. dubia, one reinstated species C. spinata Henry, 1919, and one new species C. sp. 1. Morphological characteristics were also able to accurately separate the three species. Furthermore, genetic analysis of COI sequences from Ceriodaphnia supported three clades. The high degree of correlation between morphological and molecular identification in this study indicates that mitochondrial markers, COI and 16s, are appropriate molecular markers for species discrimination and identification of Ceriodaphnia.