Differential Secondary Reconstitution of In Vivo-Selected Human SCID-Repopulating Cells in NOD/SCID versus NOD/SCID/γ chainnull Mice
Figure 5
Representative example of human multilineage engraftment in 6BG/BCNU-treated primary recipient mice. The bone marrow was harvested at 8-weeks after drug treatment, and the level of total and transduced human progenitors (CD34+), B-lymphoid (CD19+), and myeloid (CD33+) cells were determined via flow cytometry. The level of transduced cells was similar between NOD/SCID and NSG mice. The percentage in the upper right-hand quadrant represents the percentage of the human progenitor, B-lymphoid, or myeloid cells that are EGFP+.
