Breast Cancer Stem Cells Survive Periods of Farnesyl-Transferase Inhibitor-Induced Dormancy by Undergoing Autophagy
Figure 3
Effect of RhoC GTPase on JNK/SAPK activation. (a) MCF-7 cells were transiently transfected with empty vector (ctrl), a dominant active (G14V), or dominant negative (T19N) RhoC GTPase, treated with 25 M FTI or vehicle control and levels of active and total JNK/SAPK measured by western blot analysis. Shown is a representative western blot. Densitometry was performed using ImageJ and results of the ratio of active: total JNK represented as arbitrary units (AU). For significance . (b) representative western blot analysis of LC3B-I/-II levels in FTI-treated cells after inhibition of JNK/SAPK with 25 M SP600125.
