Factors Influencing the Abundance of the Side Population in a Human Myeloma Cell Line
Figure 2
SP analysis of RPMI-8226 cells maintained in culture conditions for the indicated days. (a) Cell growth curve; (b) viable cell proportion determined by PI staining by flow cytometry; (c) SP proportion over the culture day; (d) SP% change with time after subculture; (e) SP flow cytometry profiles changed over the culture day. Data are mean ± SD value of triplicate samples in an independent experiment. Bar indicates standard deviation. One of the three independent experiments is shown as representative data.