737580.fig.002a
(a) Basic Media—2 weeks
737580.fig.002b
(b) Basic Media—3 weeks
737580.fig.002c
(c) Osteogenic—2 weeks
737580.fig.002d
(d) Osteogenic—3 weeks
737580.fig.002e
(e) Adenovirus—2 weeks
737580.fig.002f
(f) Adenovirus—3 weeks
Figure 2: Von Kossa staining of cells in culture at 2 and 3 weeks. Black areas reflect the presence of mineral in the cultures. No mineralization is seen in the basic medium at 2 weeks (a) and 3 weeks (b). The noninfected rMSCs at 2 weeks (c) and 3 weeks (d) show minimal mineralization. Images of the Ad-VEGF+BMP-6 infected mixed marrow cells at 2 weeks (e) and at 3 weeks (f) showed considerable mineralization.