Selective Migration of Subpopulations of Bone Marrow Cells along an SDF-1α and ATP Gradient

Figure 5

CD34+ stem cells as well as subpopulations positive for multiple stem cell markers migrated significantly towards SDF-1 + ATP. After in vitro migration for 24 h towards medium (“spontaneous migration”), 100 ng/mL SDF-1 (“SDF-1 migration”), 100 M ATP (“ATP migration”), or a combination of both (“SDF-1 + ATP migration”), BM-TNCs were analysed by flow cytometry. Cell populations (a) and stem cell subpopulations (b) were evaluated as described in Figure 1. CD34+ cell number was significantly elevated in BM-TNCs after migration towards SDF-1 or SDF-1 + ATP (a); subpopulations expressing multiple stem cell markers also displayed higher concentrations in SDF-1 or SDF-1 + ATP migrated fraction (b), .

(a)

(b)