Combination of Complement-Dependent Cytotoxicity and Relative Fluorescent Quantification of HLA Length Polymorphisms Facilitates the Detection of a Loss of Heterozygosity

Table 1

Determination of LOH detection threshold by RFQ. Titrations series with artificial mixtures simulating (copy number neutral) loss of heterozygosity (LOH) in the HLA-A allele (for details see RFQ in material and methods).

Percentage of heterozygous DNA; (A*74/A*03)	Percentage of homozygous DNA; (A*03/A*03)	Assumed ratio of alleles; A*74 : A*03	Detected peak area ratio; A*74 : A*03
100%	0%	1 : 1,00	1 : 1,04
90%	10%	1 : 1,22	1 : 1,23
80%	20%	1 : 1,50	1 : 1,47
67%	33%	1 : 2,00	1 : 1,89
50%	50%	1 : 3,00	1 : 2,94