Table 1: Reconstitution of incomplete chondrogenesis medium.

ReagentSourceStock solution (storage)Final conc. Mix and filter sterilize

DMEM with high glucose, 1xD5671
(Sigma-Aldrich)
1x475 mL

L-Ascorbic acid 2-phosphate trisodium salt323-44822 (Wako)5 mg/mL in dH2O (4°C)50 μg/mL5 mL

L-ProlineP5607
(Sigma-Aldrich)
50 mg/mL in dH2O (4°C)50 μg/mL500 μL

Insulin-transferrin-selenium-sodium pyruvate (ITS-A) (100x)51300-044
(Gibco by Life Technologies)
1x5 mL

Sodium pyruvate (100 mM)11360-070
(Gibco by Life Technologies)
1 mM5 mL

Penicillin-streptomycin
(10,000 U/mL)
15140-122
(Gibco by Life Technologies)
100 U/mL5 mL

GlutaMAX supplement (100x) 35050-061
(Gibco by Life Technologies)
1x5 mL

Linoleic acid
(1 gm/mL)
L1012
(Sigma-Aldrich)
100 μg/μL  dilute 5 μL (1 gm/mL) to 50 μL in dH2O5.33 μg/mL26.7 μL

Dexamethasone
(MW: 392.46)
D4902, (Sigma-Aldrich)Solution-A (10 mM) (dissolve 25 mg in 6.37 mL EtOH and store at −20°C)
Solution-B (1 mM): dilute 100 μL solution-A to 1 mL in dH2O
100 nM50 μL (solution-B)