Effect of Alcohol Structure on the Optimum Condition for Novozym 435-Catalyzed Synthesis of Adipate Esters

Table 1

Range of variables for the central composite design.

Variable	Levels
	−1.682	−1.000	0.000	+1.000	+1.682
Temperature, A (°C)	35.0	41.1	50.0	58.9	65.0
Reaction time, B (min)	30.0	109.0	225.0	340.9	420.0
Enzyme amount, C (mg)	20.0	97.0	210.0	323.0	400.0
Substrate molar ratio, D	1.0	2.4	4.5	6.6	8.0