Research Article
Recovery of Bacillus licheniformis Alkaline Protease from Supernatant of Fermented Wastewater Sludge Using Ultrafiltration and Its Characterization
Table 4
Effect of enzyme inhibitor and chelator on protease activity.
| Inhibitor/chelator (5 mM) | Relative enzyme activity (%) |
| Control | 100 | β-Mercaptoethanol | 78 ± 3.8 | p-chloromercuric benzoate (p-CMB) | 91 ± 4.3 | Phenylmethyl sulphonyl fluoride (PMSF) | 0 | Diisopropyl fluorophosphate (DFP) | 10 ± 0.5 | Iodoacetate | 96 ± 4.4 | Ethelene diamine tetra acetic acid (EDTA) | 110 ± 5.6 | Dithiotheritol | 100 ± 4.8 |
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