Cloning and Expression of Randomly Mutated Bacillus
subtilis-Amylase Genes in HB101
Figure 1
PCR amplification of -amylase gene of BS168. (a) amplification of α-amylase gene. Lanes 1: DNA molecular size marker, 3: PCR product. (b) Error-prone PCR product of α-amylase gene. Lanes 1: DNA molecular size marker, 2 and 3: error-prone PCR products. 10 μL of samples were applied to a 0.7% agarose gel for electrophoretic separation.