Research ArticleCloning and Expression of Randomly Mutated Bacillus
subtilis-Amylase Genes in HB101
Figure 2
Digestion of the pET-15b containing -amylase plasmid with EcoRI restriction enzyme. Lane 1: molecular weight marker. Lanes: 2, 3, 5, 6, and 7: digested pET-15b plasmid harbouring -amylase with EcoRI restriction enzyme.