Cloning and Expression of Randomly Mutated Bacillus subtilis𝜶-Amylase Genes in HB101

Table 1

The 𝛼-amylase activity of wild, error-prone gene and empty vector.

Samples	Mean absorbance; at 540 nm	α-amylase activity; (U/mL)
Sample 3	0.306	605.39±0.0416
Sample 27	0.278	553.92±0.0134
Sample 6	0.377	735.29±0.0124
Wild-type 1	0.283	561.27±0.0015
Wild-type 2	0.271	541.67±0.0032
B. subtilis	0.0217	95.10±0.0107
Plasmid pET15-b	0.0233	97.79±0.0153