Table of Contents
Biotechnology Research International
Volume 2011, Article ID 309731, 7 pages
Research Article

Somatic Embryogenesis and Massive Shoot Regeneration from Immature Embryo Explants of Tef

1Plant Reproductive Biology, Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstraße 3, 06466 Gatersleben, Germany
2Ethiopian Institute of Agricultural Research (EIAR) Holetta, P.O. Box 2003, Addis Ababa, Ethiopia

Received 12 May 2011; Revised 7 August 2011; Accepted 8 August 2011

Academic Editor: Shengwu Ma

Copyright © 2011 Likyelesh Gugsa and Jochen Kumlehn. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. N. Vavilov, The Origin, Variation Immunity and Breeding of Cultivated Plants, The Ronald Press Co., New York, NY, USA, 1951.
  2. T. Berhe, Inheritance of Lemma color, seed color, and panicle form among four cultivars of Eragrostis tef (Zucc.) Trotter, Ph.D. thesis, University of Nebraska, Lincoln, Neb, USA, 1981.
  3. A. Tavassoli, The cytology of Eragrostis with special reference to E. tef and its relatives, Ph.D. thesis, University of London, London, UK, 1986.
  4. Central Statistical Authority (CSA), “Agricultural sample survey, 2003/04 (1996 E.C), Vol. IV,” Report on Area and Production for Major Crops (Private Peasant Holdings, Meher Season) number, Statistical Bulletin 302, Addis Ababa, Ethiopia, 2004. View at Google Scholar
  5. E. Bekele, G. Klock, and U. Zimmermann, “Somatic embryogenesis and plant regeneration from leaf and root explants and from seeds of Eragrostis tef (Gramineae),” Hereditas, vol. 123, no. 2, pp. 183–189, 1995. View at Publisher · View at Google Scholar · View at Scopus
  6. F. Mekbib, S. H. Mantell, and V. Buchanan-Wollaston, “Callus induction and in Vitro regeneration of tef [Eragrostis tef (Zucc.) Trotter] from leaf,” Journal of Plant Physiology, vol. 151, no. 3, pp. 368–372, 1997. View at Google Scholar · View at Scopus
  7. A. Kebebew, M. D. Gaj, and M. Maluszynski, “Somatic embryogenesis and plant regeneration in callus culture of tef, Eragrostis tef (Zucc.) Trotter,” Plant Cell Reports, vol. 18, no. 1-2, pp. 154–158, 1998. View at Publisher · View at Google Scholar · View at Scopus
  8. L. Gugsa, A. K. Sarial, H. Lörz, and J. Kumlehn, “Gynogenic plant regeneration from unpollinated flower explants of Eragrostis tef (Zuccagni) Trotter,” Plant Cell Reports, vol. 25, no. 12, pp. 1287–1293, 2006. View at Publisher · View at Google Scholar · View at Scopus
  9. S. Bhaskaran and R. H. Smith, “Regeneration in cereal tissue culture: a review,” Crop Science, vol. 30, pp. 1328–1337, 1990. View at Google Scholar
  10. T. Murashige and F. Skoog, “A revised medium for growth and bioassays with tobacco tissue cultures,” Plant Physiology, vol. 15, pp. 473–497, 1962. View at Google Scholar
  11. C. C. Chu, “The N6 medium and its applications to anther culture of cereal crops,” in Proceedings of the Symposium on Plant Tissue Culture, pp. 43–50, Beijing, China, 1978.
  12. J. Kumlehn, L. Serazetdinova, G. Hensel, D. Becker, and H. Loerz, “Genetic transformation of barley (Hordeum vulgare L.) via infection of androgenetic pollen cultures with Agrobacterium tumefaciens,” Plant Biotechnology Journal, vol. 4, no. 2, pp. 251–261, 2006. View at Publisher · View at Google Scholar · View at Scopus
  13. A. Jaehne, P. A. Lazzeri, M. Jaeger-Gussen, and H. Loerz, “Plant regeneration from embryonic cell suspensions derived from anther cultures of barley (Hordeum vulgare L),” Theoretical and Applied Genetics, vol. 82, pp. 74–80, 1991. View at Google Scholar
  14. K. N. Kao and M. R. Michayluk, “Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media,” Planta, vol. 126, no. 2, pp. 105–110, 1975. View at Publisher · View at Google Scholar · View at Scopus
  15. G. Hensel, V. Valkov, J. Middlefell-Williams, and J. Kumlehn, “Efficient generation of transgenic barley: the way forward to modulate plant-microbe interactions,” Journal of Plant Physiology, vol. 165, no. 1, pp. 71–82, 2008. View at Publisher · View at Google Scholar · View at Scopus
  16. J. Kumlehn and G. Hensel, “Genetic transformation technology in the Triticeae,” Breeding Science, vol. 59, no. 5, pp. 553–560, 2009. View at Publisher · View at Google Scholar · View at Scopus
  17. S. Ogita, H. Sasamoto, E. C. Yeung, and T. A. Thorpe, “The effects of glutamine on the maintenance of embryogenic cultures of Cryptomeria japonica,” In Vitro Cellular and Developmental Biology—Plant, vol. 37, no. 2, pp. 268–273, 2001. View at Google Scholar · View at Scopus
  18. A. P. Mordhorst and H. Lorz, “Embryogenesis and development of isolated barley (Hordeum vulgare L.) microspores are influenced by the amount and composition of nitrogen sources in culture media,” Journal of Plant Physiology, vol. 142, no. 4, pp. 485–492, 1993. View at Google Scholar · View at Scopus
  19. G. Hensel, C. Kastner, S. Oleszczuk, J. Riechen, and J. Kumlehn, “Agrobacterium-mediated gene transfer to cereal crop plants: current protocols for barley, wheat, triticale, and maize,” International Journal of Plant Genomics, vol. 2009, 9 pages, 2009. View at Publisher · View at Google Scholar