Somatic Embryogenesis and Massive Shoot Regeneration from Immature Embryo Explants of Tef

Table 2

The effect of 2,4-D concentration in the callus induction medium on callus formation, somatic embryogenesis, and shoot regeneration of intermediate-sized and large “Fesho” and “DZ-01-196” immature zygotic embryos. Values (within one row) followed by exclusively different superscript letters are significantly different from one another at 𝑃<0.05.

Genotype	Fesho				DZ-01-196
2,4-D [μM]	4.6	9.2	13.8	18.4	4.6	9.2	13.8	18.4
No. of cultivated embryos	35	38	36	33	43	35	43	25
% Responding embryos*	54.3^a	68.4^a	83.3^a	66.7^a	88.4^a	80.0^a	86.0^a	84.0^a
% Embryos forming somatic embryos	22.9^b	52.6^ab	66.7^a	12.1^c	37.2^a	74.3^a	81.4^a	44.0^a
% Embryos regenerating shoots	11.4^b	39.5^a	47.2^a	9.1^b	20.9^c	60.0^ab	58.1^a	8.0^b

*Embryos showing callus formation and/or somatic embryogenesis.