Somatic Embryogenesis and Massive Shoot Regeneration from Immature Embryo Explants of Tef

Table 4

The effect of immature zygotic embryo size (small: 0.1–0.2 mm, mid: 0.2–0.35 mm, large: 0.35–0.75 mm) on callus formation, somatic embryogenesis, and shoot regeneration. Values (within one row) followed by different superscript letters are significantly different from one another at 𝑃<0.05.

Genotype	Fesho			DZ-01-196
Embryo size	small	mid	large	small	mid	large
No. of cultivated embryos	65	75	75	67	69	65
% Responding embryos*	100^a	94.7^a	78.7^b	100^a	100^a	100^a
% Embryos forming somatic embryos	76.9^a	84.0^a	65.3^b	82.1^b	94.2^a	78.5^b
% Embryos regenerating shoots	55.4^b	68.0^a	41.3^c	62.7^c	88.4^a	69.2^b

*Embryos showing callus formation and/or somatic embryogenesis.