Research Article

Somatic Embryogenesis and Massive Shoot Regeneration from Immature Embryo Explants of Tef

Table 5

The effect of Phytagel concentration in the callus induction medium on the development of intermediate-sized “DZ-01-196” immature zygotic embryos. Values (within one row) followed by exclusively different superscript letters are significantly different from one another at .

Phytagel [%]0.20.30.40.50.6

No. of cultivated embryos4665677878
% Responding embryos*100a90.8a100a100a66.7a
% Embryos forming somatic embryos50.6b62.9ab95.5a100a67.3ab
% Embryos regenerating shoots20.0b45.6b82.2a100a42.1b

*Embryos showing callus formation and/or somatic embryogenesis.