Table of Contents
Biotechnology Research International
Volume 2011, Article ID 323891, 8 pages
http://dx.doi.org/10.4061/2011/323891
Research Article

Fermentative Production and Thermostability Characterization of 𝜶 Amylase from Aspergillus Species and Its Application Potential Evaluation in Desizing of Cotton Cloth

1Department of Biotechnology, Acharya Nagarjuna University, Guntur 522 510, India
2Department of Natural Sciences, Savannah State University, Savannah, GA 31404, USA
3Department of Biochemistry, National Institute of Nutrition, Tarnaka, Hyderabad 500 007, India

Received 25 June 2011; Accepted 1 August 2011

Academic Editor: Triantafyllos Roukas

Copyright © 2011 Murali Krishna Chimata et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The production of extracellular amylase was investigated employing our laboratory isolate, Aspergillus niger sp. MK 07 and effect of process variables on enzyme production, was studied in a fermentor. It was found that amylase production was maximum when the fermentor volume was maintained at 70%, rate of agitation at 250 rpm, air supply at 2.5 vvm, inoculum concentration of 10%, and a pH of 5.0. Highest enzyme production obtained under all optimized conditions was 1734 U/mL with sucrose as carbon substrate and corn steep liquor as nitrogen source. Enzyme purification studies by ammonium sulphate precipitation and Sephadex G-100 chromatography was evaluated for obtaining purified enzyme. Thermostability of amylase were evaluated with varying concentrations from 0.2 to 0.5 M concentrations of calcium chloride and the highest activity obtained was 3115 U/mL with 0.3 M calcium chloride at 55°C. Effect of temperature and pH on the activity of purified enzyme was evaluated and the purified enzyme showed an activity till 75°C and a pH of 6.5. Application potential of partially purified alpha amylase on desizing of cotton cloth was evaluated with varying enzyme concentrations from 50 to 500 U/mL and the highest desizing activity was found to be at 300 U/mL.