Placing the RPL32 Promoter Upstream of a Second Promoter Results in a Strongly Increased Number of Stably Transfected Mammalian Cell Lines That Display High Protein Expression Levels

Figure 2

d2EGFP fluorescence values in colonies induced by heterologous promoter combinations. The d2EGFP expression values were determined in stable colonies comprising DNA constructs described in Figure 1. The relative fluorescence levels were taken as arbitrary units. The average d2EGFP expression levels for each construct are indicated with a short line. The average d2EGFP expression of 637 induced by STARs 7/67/7 in the context of the TTG Zeo selection system is indicated with the horizontal line.