Placing the RPL32 Promoter Upstream of a Second Promoter Results in a Strongly Increased Number of Stably Transfected Mammalian Cell Lines That Display High Protein Expression Levels
Figure 5
Deletion analysis of the RPL32 promoter reveals critical regions. Several deletion mutants of the RPL32 promoter were created as shown at the top of the figure. The constructs were transfected to CHO-DG44 cells, up to 24 stable colonies were isolated, and d2EGFP expression values were determined. The respective colony numbers are shown above the d2EGFP expression values.
