Generation and Characterization of an scFv Directed against Site II of Rabies Glycoprotein

Figure 2

Phage ELISA showing the enrichment of phages specific for rabies virus glycoprotein during the panning cycles. After each round of panning, the output phages were added to microtiter wells with 200 ng of PV GP or bovine gelatin. Anti-M13 and helper phage was used as a control to check the back ground value. Bound phages were detected by horseradish peroxidase (HRP) conjugated anti-M13 antibody.